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N-Acetyl Cysteine Effect on Hyperlipidemia Induced Testicular Damage in Pre mature Rats
khalid M KARAM, Ahmed Saed Alebady, Khalil Gazar Chelab Al-Nailey

Last modified: 2021-09-21



Background: Prescription of antioxidants might increase the quality of sperm parameters and decrease the serum level of cholesterol and serum level of triglyceride in men who suffered from hyperlipidemia in their premature life which affected their fertility. Therefore, the present study investigated protective effects of N-Acetyl Cysteine (NAC) on sperm parameters and seminiferous tubules epithelium in high-fat diet model. Objectives: is to investigate the serum cholesterol and its effect on pre mature male rat’s fertility and evaluate the effect of treating them with N-Acetyl Cysteine (NAC).

Material and Methods: sixty pre mature male rats were divided to three groups CG (control group n=20) left without any treatment, CGF1 (n=20) was administered cholesterol for one month to induce hypercholesterolemia to reduce fertility in male rats and consequently treated with (NAC) to repair the harmful effect of cholesterol and CGF2 (n=20) was administered cholesterol only for one month. Results: cholesterol fed group were significantly different with those of control group (P<0.05) cholesterol levels were 153.51±5.39 and 93.61±4.68 mg/dl while triglyceride was 176.71±12.49 and 83.14±7.08 mg/dl in treated and control group respectively. Also semen parameters showed significant difference between treated and control group (P<0.05) they were 28.46±3.56 and 74.4±6.36×106 sperm/ml, 34.23±2.12 and 89.34±2.41%, 32.45±5.23 and3.76±1.22%, 20.34±3.81 and 3.21±1.08% for sperm count, live sperms, sperm agglutination and abnormal sperm percentage in treated and control groups respectively. While after antioxidant administration of (NAC) showed significant difference (P<0.05) with the control group in sperm count, bigger live sperms percentage and lesser abnormal sperms was (P<0.05) compared to positive control group (non NAC group), sperm agglutination had a significant difference (P<0.05) compared with positive control group (non treated group).Also, hormonal profile of  antioxidants treated group and control group was 13.14±1.07, 14.03±1.98 and 5.34±0.87 mIU/ml in G1, G2, G3, respectively for LH levels, 9.77±0.82, 10.29±0.78 and 7.23±1.46 mIU/ml in G1, G2, G3 respectively for FSH levels and 9.62±1.32, 8.35±1.03 and 7.98±1.29 ng/ml in in G1, G2, G3 respectively for testosterone levels. Conclusion: As it is shown in our study, Oral administration N-Acetyl Cysteine (NAC) with diet, decreasing the serum levels of cholesterol and serum level of TG, improving the sperm parameters by decreasing the ROS and protect the liver tissue from the harmful effect of cholesterol. finally, it seems that the antioxidants in patients with subfertility or infertility is a new and efficient strategy with few side effects.


N-Acetyl Cysteine, Cholesterol, Hyperlipidemia, Histopathology, Lipid profile, Sperm parameters.