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Tuna is a large pelagic fish that is widely found in Indonesian waters. Tuna has the potential to be processed into frozen tuna and developed into an industry. One of the things that need to be considered in the establishment of an industry is location determination. In this research, the study of determining an alternative location for the frozen tuna industry in Banda Aceh city will be done. The method used in this research is the Analytical Hierarchy Process (AHP). The criteria used in this research are Tuna Fish Production, Human Resources, Transportation Access, Institutional Support, and Availability of Infrastructure. The alternative location that will be determined is Kuta Alam Sub-district, Kuta Raja Sub-district, Meuraxa Sub-district, and Syiah Kuala Sub-district. The calculation result using the AHP method indicates that that the most appropriate location for the establishment of the frozen tuna industry is Kuta Alam Sub-districts with a weight of 0.428.