An efficient micropropagation method of hybrid napiergrass (Pennisetum purpureumn Schum) for in vitro plant production and material breeding was established from multiple-shoot clumps (MCS) regeneration system. This system was important for forage breeding system. Shoot apices from shoot-tillers produced MSC on Murashige-Skoog (MS) induction medium containing 2 mgL^–1BAP and 0.1 mgL^–12,4-D. The addition of 5 µM CuSO₄ could increase the proliferation ability of clumps. Plant regeneration was achieved by culturing on solid MS medium containing 0.1 mgL^–1NAA and 2.0 mgL^–1 BAP (51.6%). All plantlets were succesfully grown up in acclimatization stage. Based on this result, the hybrid napiergrass regeneration via MSC was a stable tissue culture system, which could be applied either for further genetic transformation assays or for alternative supply of nursery plant in the future.