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The Effect of 2,4-Dichlorophenoxyacetic acid, Benzyl Amino Purin and Cupric Sulphate on In Vitro Propagation System From Shot Apices of Shoot Tiller of Hybrid Napiergrass (Pennisetum purpureum Schum)
Nafiatul Umami, Eka Rizky Vury Rahayu, Bambang Suhartanto, Nilo Suseno

Last modified: 2021-09-28

Abstract


An efficient micropropagation method of hybrid napiergrass (Pennisetum purpureumn Schum) for in vitro plant production and material breeding was established from multiple-shoot clumps (MCS) regeneration system. This system was important for forage breeding system. Shoot apices from shoot-tillers produced MSC on Murashige-Skoog (MS) induction medium containing 2 mgL–1BAP and 0.1 mgL–12,4-D. The addition of 5 µM CuSO4 could increase the proliferation ability of clumps. Plant regeneration was achieved by culturing on solid MS medium containing 0.1 mgL–1NAA and 2.0 mgL–1 BAP (51.6%). All plantlets were succesfully grown up in acclimatization stage. Based on this result, the hybrid napiergrass regeneration via MSC was a stable tissue culture system, which could be applied either for further genetic transformation assays or for alternative supply of nursery plant in the future.


Keywords


Hybrid napiergrass; multiple-shoot formation; plant regenaration