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Effects of Heavy Metal Lead (Pb) Exposure on Chlorophyll Content and Anatomic Structure of rice (Oryza sativa L.)
Supriatno Supriatno, Chairunnisa Chairunnisa, Hafnati Rahmatan

Last modified: 2019-03-13


Lead (Pb) is one of the heavy metals that have received considerable attention as a poisonous pollutant and has a bad effect on living things, one of them on plants, especially food crops such as rice (Oryza sativa L.). Lead exposure has been shown affected on rice plant growth, but require further testing of the anatomy. This research was conducted with the aim to know the effects of heavy metal lead (Pb) on the chlorophyll and anatomy of rice, especially the anatomy of roots and leaves. The rice variety used is Inpari-32. This research type is experimental, with qualitative and quantitative approach. The experimental design used in this study was Completely Randomized Design (CRD) which consisted of three treatments variations with nine replications. Chlorophyll content was analysed by ANOVA test while root and leaf anatomy was done descriptively. From the measurement result using spectrophotometer, it showed significant differences for chlorophyll content in each treatment, where the average result of chlorophyll analysis from the three treatments were 39.31 mg/L (P0), 16.294 mg/L (P1), and 10.815 mg/L (P2). From analysis, it has been known that the values Fvalue > Ftable (27.798.532,356 > 3,40), it can be concluded that lead exposure effects total chlorophyll content and caused anatomic structures change of the roots and leaves of rice.

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