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Dengue Hemorrhagic Fever (DHF) is an infectious disease that became the leading cause of death in the tropics and subtropics Countries. Banda Aceh is one of the regions in Indonesia with the high incidence of DHF and the distribution of events per sub-district is still fluctuating, so a study of the spatial relationship of DHF distribution patterns in Banda Aceh  is needed. The aim of this study is to determine the spatial relationship of the DHF distribution pattern in Banda Aceh from 2014 to 2016. The study uses the Geographic Information System method with a case study approach. The object of this research is the entire administrative area of Banda Aceh with an analysis unit for all villages in Banda Aceh. The Moran Index Analysis was using to identification of distribution pattern by means of the Geoda application. The results showed that during the 2014 to 2016 period, the global spatial relationship of DHF incidence among Villages was only found in October and November 2014, while in other months it did not show a spatial relationship at the 95% significance level. The z value calculation for October is 2.27 November is 3,80. The local spatial association of DHF was found the villages where are 6 villages in high-high cluster, 10 villages in the low-low cluster, 2 villages in the low-high cluster, and 2 village in the high-low cluster in year 2014. Biside that, in year 2015 there were 1 village in the high-high cluster, 8 villages in the low-low cluster, 5 villages in the low-high cluster, and none in the high-low cluster. Furthermore, in year 2016 which are 3 villages in the high-high cluster, 4 villages in the low-low cluster, 5 villages in the low-high cluster, and those 1 village in the high-low cluster. Local spatial analysis found that villages in the hotspot cluster vary annually.